![]() ![]() (2008) The dynamic interplay between a cell fate determinant and a lysozyme homolog drives the asymmetric division cycle of Caulobacter crescentus. Sunish Kumar Radhakrishnan, Martin Thanbichler and Patrick H.Journal of Biological Chemistry 283, 3161-3172. (2008) The TbMTr1 spliced leader RNA cap 1 2 '-O-Ribose methyltransferase from Trypanosoma brucei acts with substrate specificity. Journal of Biological Chemistry 283, 1340-1349. (2008) Potyvirus genome-linked protein, VPg, directly affects wheat germ in vitro translation: interactions with translation initiation factors eIF4F and EIFiso4F. Journal of Experimental Botany 59, 875-889. (2008) ZmXTH1, a new xyloglucan endotransglucosylase/hydrolase in maize, affects cell wall structure and composition in Arabidopsis thaliana. (2008) Light induces peroxisome proliferation in Arabidopsis seedlings through the photoreceptor phytochrome A, the transcription factor HY5 homolog, and the peroxisomal protein peroxin11b. Shipped with Blue Ice or with Dry Ice Toxicityĭocumentation pET-28a(+) DNA - Novagen MSDS 职位 Click here for Enduser Declaration (EUD) Form. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. Please direct any questions on these use restrictions to: product contains genetically modified organisms (GMO). Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product) or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. This product is sold for internal research use only. Please contact technical service if you need additional information. Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). The pET Vectors are supplied as purified plasmid DNA (10 µg). Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map ( TB074). Tag ® configuration plus an optional C-terminal His. ![]() The pET-28a-c(+) vectors carry an N-terminal His All of the pET vectors and companion products are available as kits designed for convenient cloning, expression, detection, and purification of target proteins. T7 RNA polymerase is so selective and active that, when fully induced, almost all of the cell's resources are converted to target gene expression the desired product can comprise more than 50% of the total cell protein a few hours after induction. Target genes are cloned in pET plasmids under control of strong bacteriophage T7 transcription and (optionally) translation signals expression is induced by providing a source of T7 RNA polymerase in the host cell. ![]() The pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in E. ![]()
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